Hotspot

rpl32-trnL-UAG

SSC · 120960-122010 · 1051 bp

Back to Stachyurus

Pi 0.00647

MarkerSeek score 59.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1051
variable_sites: 12
pi: 0.00647
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	1150-1180 bp	1.0	79.9
rpl32-trnL-UAG_p2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	1151-1181 bp	1.0	79.9
rpl32-trnL-UAG_p3	GGCAGCGTTGAAAGCTTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.4	57.9	1160-1190 bp	1.0	79.0
rpl32-trnL-UAG_p4	GGCAGCGTTGAAAGCTTTTT	TTAGAAGATGCTATGCCGCC	45.0	50.0	58.4	57.8	1214-1244 bp	1.0	78.5
rpl32-trnL-UAG_p5	GGATATTGGGCAGCGTTGAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.6	58.0	1158-1188 bp	1.0	78.5