Hotspot

trnS-UGA-psbZ

LSC · 39246-39576 · 331 bp

Back to Stachyurus

Pi 0.00363

MarkerSeek score 42.30

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 3
pi: 0.00363
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	468 bp	1.0	79.9
trnS-UGA-psbZ_p2	CTATCAACCACTCGGCCATC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	457 bp	1.0	79.4
trnS-UGA-psbZ_p3	TTCACCGAGCAAAAGGAGAG	CGACCAACCATCAGGAGAAG	50.0	55.0	57.8	58.0	538 bp	1.0	79.0
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	465 bp	1.0	78.4
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	TACTCGACCAACCATCAGGA	55.0	50.0	58.1	57.8	472 bp	1.0	78.2