Hotspot

trnR-UCU-atpA

LSC · 10830-11216 · 387 bp

Back to Stachyurus

Pi 0.00155

MarkerSeek score 53.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 1
pi: 0.00155
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATCGGAATGAAAAGCGTCCA	GGACCGCTTTCTACTTCAGG	45.0	55.0	57.9	58.0	490-510 bp	1.0	83.2
trnR-UCU-atpA_p2	TCGGAATGAAAAGCGTCCAT	GGACCGCTTTCTACTTCAGG	45.0	55.0	57.9	58.0	489-509 bp	1.0	83.2
trnR-UCU-atpA_p3	ATCGGAATGAAAAGCGTCCA	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.9	58.1	534-554 bp	1.0	82.3
trnR-UCU-atpA_p4	TCGGAATGAAAAGCGTCCAT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.9	58.1	533-553 bp	1.0	82.3
trnR-UCU-atpA_p5	TCGGAATGAAAAGCGTCCAT	CATTCACCGAGGAAGCAGAA	45.0	50.0	57.9	57.9	541-561 bp	1.0	82.1