Hotspot

psbI-trnS-GCU

LSC · 8784-8969 · 186 bp

Back to Stachyurus

Pi 0.0213

MarkerSeek score 59.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 7
pi: 0.0213
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGGCGTGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.6	58.0	273-288 bp	1.0	81.3
psbI-trnS-GCU_p2	GTAATCCTGGGCGTGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.6	58.0	274-289 bp	1.0	81.3
psbI-trnS-GCU_p3	GTAATCCTGGGCGTGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.6	58.1	292-307 bp	1.0	80.2
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.6	58.4	271-286 bp	1.0	78.5
psbI-trnS-GCU_p5	GTAATCCTGGGCGTGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.6	58.4	278-293 bp	1.0	78.3