Hotspot

psbK-psbI

LSC · 8352-8672 · 321 bp

Back to Stachyurus

Pi 0.00374

MarkerSeek score 36.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 321
variable_sites: 2
pi: 0.00374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCAAATTACCCGAGGCCTAC	TTCTTCACGCCCAGGATTAC	55.0	50.0	58.0	57.6	544-545 bp	1.0	77.0
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	458-459 bp	1.0	76.9
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	455-456 bp	1.0	74.9
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	456-457 bp	1.0	74.9
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	457-458 bp	1.0	74.9