Hotspot

trnH-GUG-psbA

LSC · 196-602 · 407 bp

Back to Stachyurus

Pi 0.00246

MarkerSeek score 34.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 2
pi: 0.00246
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	472-473 bp	1.0	78.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	486-487 bp	1.0	78.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	485-486 bp	1.0	78.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	487-488 bp	1.0	78.9
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	475-476 bp	1.0	78.9