Hotspot

ndhD

SSC · 111448-112968 · 1521 bp

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Pi 0.00951

MarkerSeek score 47.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 41
pi: 0.00951
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGGCCCTTTTATGTGGATCA	AGGACTGCGTTGGTTGTAAG	50.0	50.0	57.9	58.1	80-2447 bp	1.0	82.0
ndhD_p2	CGGCCCTTTTATGTGGATCA	CCCTGGGATGGATGTAAAGC	50.0	55.0	57.9	58.0	80-2494 bp	0.7	70.3
ndhD_p3	AGTTGGGACCCAAAGGAAAC	CCCTGGGATGGATGTAAAGC	50.0	55.0	57.9	58.0	80-2174 bp	0.7	70.0
ndhD_p4	GTTGGGACCCAAAGGAAACT	CCCTGGGATGGATGTAAAGC	50.0	55.0	57.9	58.0	80-2173 bp	0.7	70.0
ndhD_p5	GAAGCGTGGGGATCCTATTG	CCCTGGGATGGATGTAAAGC	55.0	55.0	58.1	58.0	80-2195 bp	0.7	69.8