Hotspot

rps15-ycf1

SSC · 119244-119632 · 389 bp

Back to Stachys

Pi 0.0371

MarkerSeek score 60.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 389
variable_sites: 35
pi: 0.0371
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ATCCGTTGACGTTTTCCCAA	AGCAGCCAAGTTTCACATGA	45.0	45.0	58.0	58.0	80-775 bp	1.0	85.1
rps15-ycf1_p2	CAGCATCCGTTGACGTTTTC	AGCAGCCAAGTTTCACATGA	50.0	45.0	58.0	58.0	80-779 bp	1.0	84.9
rps15-ycf1_p3	TGACAAATAGGCCAGCATCC	AGCAGCCAAGTTTCACATGA	50.0	45.0	57.9	58.0	80-791 bp	1.0	84.8
rps15-ycf1_p4	ATCCGTTGACGTTTTCCCAA	GCAGCCAAGTTTCACATGAC	45.0	50.0	58.0	57.9	80-774 bp	1.0	84.8
rps15-ycf1_p5	AACACCATGCATTGATGGGT	AGCAGCCAAGTTTCACATGA	45.0	45.0	58.1	58.0	80-1035 bp	1.0	83.9