Hotspot

ndhG-ndhI

SSC · 114615-114989 · 375 bp

Back to Stachys

Pi 0.0296

MarkerSeek score 62.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 375
variable_sites: 27
pi: 0.0296
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAACCGTCCAAAGATGAA	GAGGCCGAATCCACTTTGAA	50.0	50.0	58.1	58.2	80-1010 bp	1.0	83.1
ndhG-ndhI_p2	ATGATCCCATACCAGGACGT	GAGGCCGAATCCACTTTGAA	50.0	50.0	57.9	58.2	80-1093 bp	1.0	82.6
ndhG-ndhI_p3	CCCAACCGTCCAAAGATGAA	CTGCCAGGTACATTGGTCAA	50.0	50.0	58.1	57.8	80-1118 bp	1.0	82.6
ndhG-ndhI_p4	CCCAACCGTCCAAAGATGAA	TGCCAGGTACATTGGTCAAG	50.0	50.0	58.1	57.8	80-1117 bp	1.0	82.6
ndhG-ndhI_p5	ATGATCCCATACCAGGACGT	TGCCAGGTACATTGGTCAAG	50.0	50.0	57.9	57.8	80-1200 bp	1.0	82.2