Hotspot

ndhD-psaC

SSC · 112969-113071 · 103 bp

Back to Stachys

Pi 0.0624

MarkerSeek score 67.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 103
variable_sites: 14
pi: 0.0624
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GCAAGGAAAATGGTTCGTGG	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.9	57.7	80-233 bp	1.0	82.4
ndhD-psaC_p2	GCAAGGAAAATGGTTCGTGG	GAGATGTGAATCCGCCTGTC	50.0	55.0	57.9	58.4	80-231 bp	1.0	81.7
ndhD-psaC_p3	GCAAGGAAAATGGTTCGTGG	ATGGCATGAAACAACTCGCA	50.0	45.0	57.9	58.8	80-180 bp	1.0	80.5
ndhD-psaC_p4	GCAAGGAAAATGGTTCGTGG	AGAGATGTGAATCCGCCTGT	50.0	50.0	57.9	58.8	80-232 bp	1.0	78.6
ndhD-psaC_p5	GCAAGGAAAATGGTTCGTGG	TGTAAGAGATGTGAATCCGCC	50.0	47.6	57.9	57.8	80-236 bp	1.0	75.5