Hotspot

ccsA-ndhD

SSC · 111267-111447 · 181 bp

Back to Stachys

Pi 0.0418

MarkerSeek score 67.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 181
variable_sites: 19
pi: 0.0418
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGGCCCTTTTATGTGGATCA	GATATGGGACTCAACGGAGC	50.0	55.0	57.9	57.8	80-1243 bp	1.0	82.2
ccsA-ndhD_p2	CGGCCCTTTTATGTGGATCA	CTCAACGGAGCCCTTTTACA	50.0	50.0	57.9	57.8	80-1234 bp	1.0	82.1
ccsA-ndhD_p3	CGGCCCTTTTATGTGGATCA	ACTCAACGGAGCCCTTTTAC	50.0	50.0	57.9	57.8	80-1235 bp	1.0	82.1
ccsA-ndhD_p4	TTGGCCCGAGTGCTATTTTT	GATATGGGACTCAACGGAGC	45.0	55.0	58.1	57.8	80-1381 bp	1.0	81.9
ccsA-ndhD_p5	CGGCCCTTTTATGTGGATCA	TCTCTCGGTCAACGCAATTT	50.0	45.0	57.9	57.8	80-1333 bp	0.8	73.5