Hotspot

psaA-ycf3

LSC · 40383-41131 · 749 bp

Back to Stachys

Pi 0.0138

MarkerSeek score 60.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 29
pi: 0.0138
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAG	50.0	50.0	57.9	58.1	80-849 bp	1.0	85.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	80-856 bp	1.0	84.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	80-860 bp	1.0	84.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	80-855 bp	1.0	83.8
psaA-ycf3_p5	TTTGGCCCATTCCTCGAAAG	TCACGAGGCGTTTCGAATAG	50.0	50.0	58.5	58.1	80-853 bp	1.0	82.6