Hotspot

trnE-UUC-trnT-GGU

LSC · 29935-30504 · 570 bp

Back to Stachys

Pi 0.016

MarkerSeek score 63.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 22
pi: 0.016
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	80-727 bp	1.0	80.9
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	80-706 bp	1.0	80.6
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	80-703 bp	1.0	80.4
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	80-724 bp	1.0	79.8
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TTGAACCGATGACTTACGCC	55.0	50.0	58.3	58.3	80-729 bp	1.0	78.7