Hotspot

rpoB-trnC-GCA

LSC · 26303-27403 · 1101 bp

Back to Stachys

Pi 0.0179

MarkerSeek score 54.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 50
pi: 0.0179
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	AACGGGGCTTCACAATCTTT	50.0	45.0	58.0	58.0	80-1846 bp	1.0	84.3
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	ACGGGGCTTCACAATCTTTT	50.0	45.0	58.0	58.0	80-1845 bp	1.0	84.3
rpoB-trnC-GCA_p3	CCGCCTACACAAGCAAATTG	AACGGGGCTTCACAATCTTT	50.0	45.0	58.0	58.0	80-2038 bp	1.0	84.1
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	ACGGGGCTTCACAATCTTTT	50.0	45.0	58.0	58.0	80-2037 bp	1.0	84.1
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	GAAGGGAATCGCGAAGTCTT	50.0	50.0	58.0	58.0	80-2112 bp	1.0	83.9