Hotspot

rps15-ycf1

SSC · 130495-131043 · 549 bp

Back to Stachyphrynium

Pi 0.0857

MarkerSeek score 79.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 549
variable_sites: 43
pi: 0.0857
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ATAAGCCAGCATACGTCGAC	ACACTGGACAACATGGCAAT	50.0	45.0	57.9	58.0	1133-1185 bp	1.0	86.6
rps15-ycf1_p2	AACACCATGCATTGATGGGT	ACACTGGACAACATGGCAAT	45.0	45.0	58.1	58.0	1384-1436 bp	1.0	86.4
rps15-ycf1_p3	GGCTCAGCTCCAGCATAATT	ACACTGGACAACATGGCAAT	50.0	45.0	58.0	58.0	1637-1689 bp	1.0	85.8
rps15-ycf1_p4	GAAGCTATACGGCTCAGCTC	ACACTGGACAACATGGCAAT	55.0	45.0	57.9	58.0	1647-1699 bp	1.0	85.5
rps15-ycf1_p5	GAGAAGCTATACGGCTCAGC	ACACTGGACAACATGGCAAT	55.0	45.0	57.9	58.0	1649-1701 bp	1.0	85.5