Hotspot

ndhG-ndhI

SSC · 125640-126140 · 501 bp

Back to Stachyphrynium

Pi 0.1

MarkerSeek score 81.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 501
variable_sites: 48
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TACTACCCCCAGACTCCCTA	TCAATTACGTCGGAGCGTTT	55.0	45.0	57.7	57.9	981-989 bp	1.0	83.3
ndhG-ndhI_p2	TGAAGTGACTCCATCCCCAA	TCAATTACGTCGGAGCGTTT	50.0	45.0	58.3	57.9	1203-1211 bp	1.0	82.7
ndhG-ndhI_p3	GGTCCTGTTCGATGATCTGG	TCAATTACGTCGGAGCGTTT	55.0	45.0	57.8	57.9	1309 bp	0.5	57.6
ndhG-ndhI_p4	GGTCCTGTTCGATGATCTGG	TGCGAAGTATGTGTTCGTGT	55.0	45.0	57.8	57.9	1249 bp	0.5	57.0
ndhG-ndhI_p5	GGTCCTGTTCGATGATCTGG	GGTGACCGGGTTCATGAATT	55.0	50.0	57.8	58.2	1436 bp	0.5	56.8