Hotspot

ndhF-rpl32

SSC · 118367-119353 · 987 bp

Back to Stachyphrynium

Pi 0.0584
MarkerSeek score 79.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
987
variable_sites
54
pi
0.0584
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGCATATTGATATGTATGTTCCA GGTGGAAACTGATTTCGCTA 30.4 45.0 53.7 55.8 1131-1139 bp 1.0 47.8
ndhF-rpl32_p2 TGCATATTGATATGTATGTTCCA GGTGGAAACTGATTTCGCTAA 30.4 42.9 53.7 56.5 1131-1139 bp 1.0 47.8
ndhF-rpl32_p3 ATGCATATTGATATGTATGTTCCA GGTGGAAACTGATTTCGCTA 29.2 45.0 54.3 55.8 1132-1140 bp 1.0 47.8
ndhF-rpl32_p4 TGCATATTGATATGTATGTTCCAT GGTGGAAACTGATTTCGCTA 29.2 45.0 54.3 55.8 1131-1139 bp 1.0 47.8
ndhF-rpl32_p5 TGCATATTGATATGTATGTTCCA GGTGGAAACTGATTTCGCTAAA 30.4 40.9 53.7 57.2 1131-1139 bp 1.0 47.8