Hotspot

rpl16-rps3

LSC · 87263-87414 · 152 bp

Back to Stachyphrynium

Pi 0.0486

MarkerSeek score 72.00

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 7
pi: 0.0486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	TGGAGTATTAGGCATCCAAA	45.0	40.0	57.1	53.7	211-219 bp	1.0	45.6
rpl16-rps3_p2	ACGAGTCACACACTAAGCAT	TGGAGTATTAGGCATCCAAA	45.0	40.0	56.9	53.7	210-218 bp	1.0	45.6
rpl16-rps3_p3	AACGAGTCACACACTAAGCA	ATGGAGTATTAGGCATCCAA	45.0	40.0	57.1	53.4	212-220 bp	1.0	45.6
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	TGGAGTATTAGGCATCCAAAT	45.0	38.1	57.1	54.4	211-219 bp	1.0	45.6
rpl16-rps3_p5	AACGAGTCACACACTAAGCA	TCTATGGAGTATTAGGCATCCA	45.0	40.9	57.1	55.6	215-223 bp	1.0	45.5