Hotspot

psbB-psbT

LSC · 78496-78687 · 192 bp

Back to Stachyphrynium

Pi 0.0729

MarkerSeek score 71.70

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 14
pi: 0.0729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TGGAGATCCAACTACAAGGAGA	CCCTAAAGTCGAGACTAAAAGGA	45.5	43.5	57.9	57.3	271-282 bp	1.0	44.1
psbB-psbT_p2	GGAGATCCAACTACAAGGAGAC	CCCTAAAGTCGAGACTAAAAGGA	50.0	43.5	57.6	57.3	270-281 bp	1.0	44.1
psbB-psbT_p3	GGAGATCCAACTACAAGGAGAC	TCCCTAAAGTCGAGACTAAAAGG	50.0	43.5	57.6	57.3	271-282 bp	1.0	44.1
psbB-psbT_p4	TGGAGATCCAACTACAAGGAGA	CCCTAAAGTCGAGACTAAAAGGAA	45.5	41.7	57.9	58.0	271-282 bp	1.0	44.1
psbB-psbT_p5	TGGAGATCCAACTACAAGGAGA	TCCCTAAAGTCGAGACTAAAAGG	45.5	43.5	57.9	57.3	272-283 bp	1.0	44.0