Hotspot

rbcL-accD

LSC · 60529-61293 · 765 bp

Back to Stachyphrynium

Pi 0.0419
MarkerSeek score 72.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
765
variable_sites
32
pi
0.0419
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TCGAATTTGAACCAGTAGATAAGC ACATCGAATTGAACCGCCAT 37.5 45.0 57.1 58.2 837-946 bp 1.0 43.1
rbcL-accD_p2 CGAATTTGAACCAGTAGATAAGCT ACATCGAATTGAACCGCCAT 37.5 45.0 57.1 58.2 836-945 bp 1.0 43.1
rbcL-accD_p3 TCGAATTTGAACCAGTAGATAAGC TTGAACCGCCATTTTCCCAT 37.5 45.0 57.1 58.4 829-938 bp 1.0 43.1
rbcL-accD_p4 TCGAATTTGAACCAGTAGATAAGC CAACATCGAATTGAACCGCC 37.5 50.0 57.1 58.1 839-948 bp 1.0 43.0
rbcL-accD_p5 CGAATTTGAACCAGTAGATAAGCT CAACATCGAATTGAACCGCC 37.5 50.0 57.1 58.1 838-947 bp 1.0 43.0