Hotspot

trnD-GUC-trnY-GUA

LSC · 32679-33086 · 408 bp

Back to Stachyphrynium

Pi 0.0444

MarkerSeek score 73.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 17
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	AATTCTTCCTGGGTCGATGC	50.0	50.0	58.5	58.0	520-532 bp	1.0	82.2
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	487-499 bp	1.0	80.1
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	486-498 bp	1.0	79.9
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.5	57.5	482-494 bp	1.0	78.8
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGACTGTAAATTCGTTGGCG	50.0	50.0	58.5	57.5	481-493 bp	1.0	78.8