Hotspot

trnS-GCU-trnG-UCC

LSC · 8831-10253 · 1423 bp

Back to Stachyphrynium

Pi 0.0929
MarkerSeek score 83.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1423
variable_sites
126
pi
0.0929
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GCTTTAGTCCACTCAGCCAT AAAACGGAGAGACCCCCTAA 50.0 50.0 57.6 58.0 1523-1724 bp 1.0 86.0
trnS-GCU-trnG-UCC_p2 GCTTTAGTCCACTCAGCCAT AAAAACGGAGAGACCCCCTA 50.0 50.0 57.6 58.0 1524-1725 bp 1.0 85.9
trnS-GCU-trnG-UCC_p3 AGTCCACTCAGCCATCTCTC AAAAACGGAGAGACCCCCTA 55.0 50.0 58.5 58.0 1519-1720 bp 1.0 85.1
trnS-GCU-trnG-UCC_p4 AGTCCACTCAGCCATCTCTC AAAACGGAGAGACCCCCTAA 55.0 50.0 58.5 58.0 1518-1719 bp 1.0 85.1
trnS-GCU-trnG-UCC_p5 TAGTCCACTCAGCCATCTCT AAAACGGAGAGACCCCCTAA 50.0 50.0 57.2 58.0 1519-1720 bp 1.0 82.6