Hotspot

matK-trnK-UUU

LSC · 3298-4242 · 945 bp

Back to Stachyphrynium

Pi 0.0489

MarkerSeek score 77.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 945
variable_sites: 45
pi: 0.0489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGAAAGTGTTGTTGCCGAGA	TCGGGTTGCTAACTCAATGG	45.0	50.0	57.7	57.9	1040-1094 bp	1.0	81.7
matK-trnK-UUU_p2	AGAAAGTGTTGTTGCCGAGA	CGGGTTGCTAACTCAATGGT	45.0	50.0	57.7	58.2	1039-1093 bp	1.0	81.1
matK-trnK-UUU_p3	AAGTGTTGTTGCCGAGATCT	TCGGGTTGCTAACTCAATGG	45.0	50.0	57.4	57.9	1037-1091 bp	1.0	79.9
matK-trnK-UUU_p4	AAGTGTTGTTGCCGAGATCT	CGGGTTGCTAACTCAATGGT	45.0	50.0	57.4	58.2	1036-1090 bp	1.0	79.4
matK-trnK-UUU_p5	AAAGTGTTGTTGCCGAGATCT	TCGGGTTGCTAACTCAATGG	42.9	50.0	58.1	57.9	1038-1092 bp	1.0	75.1