Hotspot

rps19

LSC · 160919-161141 · 223 bp

Back to Spyridium

Pi 0.0185

MarkerSeek score 65.10

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 223
variable_sites: 25
pi: 0.0185
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTGATTCTTCGTCGCCGTAG	AGCTAGATAACTAGAAAAACAAAA	50.0	25.0	58.1	52.4	80-273 bp	0.977273	49.4
rps19_p2	GGGCCCCAATTGGTAGAAAA	AGCTAGATAACTAGAAAAACAAAA	50.0	25.0	58.1	52.4	80-365 bp	0.977273	47.6
rps19_p3	CCGGATCGAAATGTTGGCTA	AGCTAGATAACTAGAAAAACAAAA	50.0	25.0	58.1	52.4	80-455 bp	0.977273	45.9
rps19_p4	AAAAGTTTGGGTAGAGCCGG	AGCTAGATAACTAGAAAAACAAAA	50.0	25.0	58.1	52.4	80-471 bp	0.977273	45.6
rps19_p5	ATTACCTTCTGGGGAGGTCC	AGCTAGATAACTAGAAAAACAAAA	55.0	25.0	58.1	52.4	80-553 bp	0.977273	44.6