Hotspot

rps15-ycf1

SSC · 129661-130035 · 375 bp

Back to Spyridium

Pi 0.0102

MarkerSeek score 58.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 375
variable_sites: 29
pi: 0.0102
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ATTGGTGAGGCTGCGTATTT	TGGCAGTCGTTTTAGTCTGG	45.0	50.0	57.9	57.8	80-527 bp	1.0	88.1
rps15-ycf1_p2	ATTGGTGAGGCTGCGTATTT	GGCAGTCGTTTTAGTCTGGT	45.0	50.0	57.9	57.8	80-526 bp	1.0	87.9
rps15-ycf1_p3	CTATTGGTGAGGCTGCGTAT	TGGCAGTCGTTTTAGTCTGG	50.0	50.0	57.5	57.8	80-529 bp	1.0	84.9
rps15-ycf1_p4	CTATTGGTGAGGCTGCGTAT	GGCAGTCGTTTTAGTCTGGT	50.0	50.0	57.5	57.8	80-528 bp	1.0	84.8
rps15-ycf1_p5	GGTGAGGCTGCGTATTTGAA	TGGCAGTCGTTTTAGTCTGG	50.0	50.0	58.6	57.8	80-524 bp	1.0	84.6