Hotspot

ycf1-ndhF

SSC · 115448-115513 · 66 bp

Back to Spyridium

Pi 0.0863

MarkerSeek score 63.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 66
variable_sites: 32
pi: 0.0863
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GCAGAATACCGTCACCCATT	GGCGGTCGAATTTCTTCCTA	50.0	50.0	58.0	57.7	80-690 bp	1.0	86.3
ycf1-ndhF_p2	GCAGAATACCGTCACCCATT	GAATGGTGTCGGTATTGCGA	50.0	50.0	58.0	58.4	80-745 bp	1.0	85.9
ycf1-ndhF_p3	GCAGAATACCGTCACCCATT	AATGGTGTCGGTATTGCGAG	50.0	50.0	58.0	58.3	80-744 bp	0.977273	85.1
ycf1-ndhF_p4	GCAGAATACCGTCACCCATT	ACACTTTCTCATATCCGCCG	50.0	50.0	58.0	57.8	80-1253 bp	0.931818	83.4
ycf1-ndhF_p5	AGGGGGACTAAACAGGAACA	ACACTTTCTCATATCCGCCG	50.0	50.0	57.9	57.8	80-1140 bp	0.931818	83.0