Hotspot

rpoB-trnC-GCA

LSC · 28634-29695 · 1062 bp

Back to Spyridium

Pi 0.00437

MarkerSeek score 58.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 39
pi: 0.00437
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCTCAGTTCTACCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1144-1160 bp	1.0	82.5
rpoB-trnC-GCA_p2	TCCTCAGTTCTACCCCCAAG	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	58.4	1126-1142 bp	1.0	80.4
rpoB-trnC-GCA_p3	TCAGTTCTACCCCCAAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.3	58.1	1141-1157 bp	1.0	80.3
rpoB-trnC-GCA_p4	TTCCTCAGTTCTACCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.6	58.1	1145-1161 bp	1.0	79.5
rpoB-trnC-GCA_p5	TTTCCTCAGTTCTACCCCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.6	58.1	1146-1162 bp	1.0	79.5