Hotspot

rps2-rpoC2

LSC · 18032-18260 · 229 bp

Back to Spyridium

Pi 0.0113

MarkerSeek score 60.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 15
pi: 0.0113
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GTGCCATTCGAGGATTCCAT	TCGAGTGGGAAATGAAAGACA	50.0	42.9	58.0	57.2	437-451 bp	1.0	71.9
rps2-rpoC2_p2	AACTCCTGCTCCCATCATCT	TCGAGTGGGAAATGAAAGACA	50.0	42.9	58.1	57.2	394-408 bp	1.0	71.9
rps2-rpoC2_p3	TGCTCCCATCATCTCTTCCA	TCGAGTGGGAAATGAAAGACA	50.0	42.9	58.1	57.2	388-402 bp	1.0	71.9
rps2-rpoC2_p4	AAGGTGCCATTCGAGGATTC	TCGAGTGGGAAATGAAAGACA	50.0	42.9	58.0	57.2	440-454 bp	1.0	71.7
rps2-rpoC2_p5	TCCTGCTCCCATCATCTCTT	TCGAGTGGGAAATGAAAGACA	50.0	42.9	57.8	57.2	391-405 bp	1.0	71.3