Hotspot

atpH-atpI

LSC · 15116-16356 · 1241 bp

Back to Spyridium

Pi 0.00331

MarkerSeek score 62.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 37
pi: 0.00331
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1246-1368 bp	1.0	85.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1212-1334 bp	1.0	85.8
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	50.0	45.0	58.0	57.9	1276-1398 bp	1.0	84.9
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.0	57.9	1242-1364 bp	1.0	84.9
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	57.9	58.0	1228-1350 bp	1.0	84.8