Hotspot

ycf2

IRa · 149598-155867 · 6270 bp

Back to Spuriopimpinella

Pi 0.0303

MarkerSeek score 43.00

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6270
variable_sites: 17
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.09
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CAAAGATCTTGAGCGACCGA	TTATCTTTGTTTCCCGGGCC	50.0	50.0	58.0	58.2	1840 bp	0.5	58.7
ycf2_p2	CAAAGATCTTGAGCGACCGA	CTTACTTCGACAGGGCACAA	50.0	50.0	58.0	57.8	2222 bp	0.5	58.7
ycf2_p3	CAAAGATCTTGAGCGACCGA	ACGTTGAGAAAGGGCAGATG	50.0	50.0	58.0	58.2	2320 bp	0.5	58.5
ycf2_p4	CAAAGATCTTGAGCGACCGA	CGTTGAGAAAGGGCAGATGT	50.0	50.0	58.0	58.2	2319 bp	0.5	58.5
ycf2_p5	ATTTCTTTCATCGGGTCCGG	TTATCTTTGTTTCCCGGGCC	50.0	50.0	58.0	58.2	1868 bp	0.5	58.4