Hotspot

ndhF-rpl32

SSC · 116504-117632 · 1129 bp

Back to Spuriopimpinella

Pi 0.066

MarkerSeek score 75.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1129
variable_sites: 60
pi: 0.066
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	1842-1891 bp	1.0	88.7
ndhF-rpl32_p2	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	2021-2070 bp	1.0	88.6
ndhF-rpl32_p3	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	2020-2069 bp	1.0	88.6
ndhF-rpl32_p4	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	2249-2298 bp	1.0	88.1
ndhF-rpl32_p5	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	2248-2297 bp	1.0	88.1