Hotspot

trnW-CCA-trnP-UGG

LSC · 70392-70524 · 133 bp

Back to Spuriopimpinella

Pi 0.0846

MarkerSeek score 78.10

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 11
pi: 0.0846
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	220-222 bp	1.0	83.7
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	219-221 bp	1.0	83.2
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	212-214 bp	1.0	83.2
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	247-249 bp	1.0	82.9
trnW-CCA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	246-248 bp	1.0	82.5