Hotspot

psbE-petL

LSC · 65544-69826 · 4283 bp

Back to Spuriopimpinella

Pi 0.0798

MarkerSeek score 58.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 4283
variable_sites: 79
pi: 0.0798
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.23
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TTACATTGTAGTCGGTGGCG	GGACCAGCCACACAACTAAA	50.0	50.0	58.0	58.0	1829 bp	0.5	59.8
psbE-petL_p2	GGAAGGGAAAGTGAGTTGCA	GGACCAGCCACACAACTAAA	50.0	50.0	58.0	58.0	1702 bp	0.5	59.7
psbE-petL_p3	TTACATTGTAGTCGGTGGCG	GAATGCGGTCCCTTATTCGT	50.0	50.0	58.0	58.1	1593 bp	0.5	59.6
psbE-petL_p4	TTACATTGTAGTCGGTGGCG	GCGTCTGCTATCATCTCAGG	50.0	55.0	58.0	57.9	1567 bp	0.5	59.5
psbE-petL_p5	GGAAGGGAAAGTGAGTTGCA	GAATGCGGTCCCTTATTCGT	50.0	50.0	58.0	58.1	1466 bp	0.5	59.4