Hotspot

trnE-UUC-trnT-GGU

LSC · 31380-32130 · 751 bp

Back to Spuriopimpinella

Pi 0.0974

MarkerSeek score 87.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 68
pi: 0.0974
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	855 bp	1.0	89.0
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	820 bp	1.0	88.4
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	852 bp	1.0	87.8
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	857 bp	1.0	86.7
trnE-UUC-trnT-GGU_p5	TTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	57.4	58.0	890 bp	1.0	85.6