Hotspot

rps16-trnQ-UUG

LSC · 5720-6957 · 1238 bp

Back to Spuriopimpinella

Pi 0.0555
MarkerSeek score 77.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1238
variable_sites
56
pi
0.0555
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 1321-1336 bp 1.0 82.8
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 1322-1337 bp 1.0 82.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.1 58.5 1332-1347 bp 1.0 82.0
rps16-trnQ-UUG_p4 TGCTTTCTACCACAGCGTTT TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.0 57.3 1303-1318 bp 1.0 81.8
rps16-trnQ-UUG_p5 TTGCTTTCTACCACAGCGTT TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.0 57.3 1304-1319 bp 1.0 81.8