Hotspot

psbA-trnK-UUU

LSC · 1326-1540 · 215 bp

Back to Spuriopimpinella

Pi 0.115

MarkerSeek score 82.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 23
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.8	57.8	317-332 bp	1.0	85.7
psbA-trnK-UUU_p2	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.7	57.8	318-333 bp	1.0	85.1
psbA-trnK-UUU_p3	TACAGAAGCGACCCCATAGG	CTAGTTCCGGGTTCGAATCC	55.0	55.0	58.6	57.8	286-301 bp	1.0	83.8
psbA-trnK-UUU_p4	TTTTCGGTGCTGGTTATCCA	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.4	57.8	308-323 bp	1.0	83.3
psbA-trnK-UUU_p5	TAAAGACGGTTTTCGGTGCT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.8	58.6	328-343 bp	1.0	82.2