Hotspot

ycf1

SSC · 123992-129346 · 5355 bp

Back to Spryginia

Pi 0.00324

MarkerSeek score 46.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5355
variable_sites: 26
pi: 0.00324
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAGCTGGTTCAGGATTTCT	AGAAACCTTGGGTTATACCA	45.0	40.0	56.1	53.5	217 bp	1.0	41.5
ycf1_p2	AGAGCTGGTTCAGGATTTCT	AAGAAACCTTGGGTTATACCA	45.0	38.1	56.1	54.4	218 bp	1.0	41.5
ycf1_p3	AGAGCTGGTTCAGGATTTCT	AGAAACCTTGGGTTATACCAA	45.0	38.1	56.1	54.4	217 bp	1.0	41.5
ycf1_p4	TGGTTTGATAGAGCTGGTTCAG	AGAAACCTTGGGTTATACCA	45.5	40.0	58.1	53.5	226 bp	1.0	41.4
ycf1_p5	TGGTTTGATAGAGCTGGTTCA	AGAAACCTTGGGTTATACCA	42.9	40.0	56.9	53.5	226 bp	1.0	41.4