Hotspot

ndhK

LSC · 49211-49888 · 678 bp

Back to Spryginia

Pi 0.00197

MarkerSeek score 60.00

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 2
pi: 0.00197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	ACAAAGTGCCCTGCATCTTT	ATGGCGAAAGGGAGCATTAG	45.0	50.0	58.3	58.0	888-889 bp	1.0	77.6
ndhK_p2	ACAAAGTGCCCTGCATCTTT	GGCGAAAGGGAGCATTAGAA	45.0	50.0	58.3	58.0	886-887 bp	1.0	77.5
ndhK_p3	ACAAAGTGCCCTGCATCTTT	TTTATGCATGGCGAAAGGGA	45.0	45.0	58.3	57.9	895-896 bp	1.0	76.8
ndhK_p4	ACAAAGTGCCCTGCATCTTT	GTTTATGCATGGCGAAAGGG	45.0	50.0	58.3	57.8	896-897 bp	1.0	76.2
ndhK_p5	ACAAAGTGCCCTGCATCTTT	CATGGCGAAAGGGAGCATTA	45.0	50.0	58.3	58.3	889-890 bp	1.0	75.3