Hotspot

ndhF-rpl32

SSC · 112559-113411 · 853 bp

Back to Spryginia

Pi 0.00548

MarkerSeek score 64.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 853
variable_sites: 7
pi: 0.00548
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAAGGGA	ACGATGTCCAATATCCCTTCC	47.6	47.6	57.6	57.2	977-990 bp	1.0	56.3
ndhF-rpl32_p2	TTGTTGCTGTCGGAAAAAGT	ACGATGTCCAATATCCCTTCC	40.0	47.6	56.4	57.2	1025-1038 bp	1.0	54.9
ndhF-rpl32_p3	TGGAAGTGGAATGAAAGGGA	ACGATGTCCAATATCCCTTCC	45.0	47.6	56.3	57.2	976-989 bp	1.0	54.5
ndhF-rpl32_p4	CTGGAAGTGGAATGAAAGGGA	CGATGTCCAATATCCCTTCCT	47.6	47.6	57.6	56.9	976-989 bp	1.0	54.1
ndhF-rpl32_p5	CTGGAAGTGGAATGAAAGGGA	AACGATGTCCAATATCCCTTCC	47.6	45.5	57.6	57.9	978-991 bp	1.0	53.8