Hotspot

psaJ-rpl33

LSC · 66809-67233 · 425 bp

Back to Spryginia

Pi 0.0125

MarkerSeek score 63.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 8
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTAGCGCC	ATATCTGGAAATTCCCGCCG	50.0	50.0	57.8	57.8	638-640 bp	1.0	78.3
psaJ-rpl33_p2	TAGCGCCGGTACTAAGTACT	ATATCTGGAAATTCCCGCCG	50.0	50.0	57.7	57.8	625-627 bp	1.0	76.9
psaJ-rpl33_p3	ACATATCTTTCCGTAGCGCC	AATTCCCGCCGATTCTTTCT	50.0	45.0	57.8	57.6	629-631 bp	1.0	76.5
psaJ-rpl33_p4	ACATATCTTTCCGTAGCGCC	ATTCCCGCCGATTCTTTCTT	50.0	45.0	57.8	57.6	628-630 bp	1.0	76.5
psaJ-rpl33_p5	GTAGCGCCGGTACTAAGTAC	ATATCTGGAAATTCCCGCCG	55.0	50.0	57.6	57.8	626-628 bp	1.0	76.4