Hotspot

rbcL-accD

LSC · 56156-56831 · 676 bp

Back to Spryginia

Pi 0.00986

MarkerSeek score 58.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 10
pi: 0.00986
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTTGTGAAGTGTGGAAGGA	TCGCCTGTGTATTCCGATTC	50.0	50.0	58.0	57.8	804-809 bp	1.0	79.1
rbcL-accD_p2	GCTTGTGAAGTGTGGAAGGA	CGCCTGTGTATTCCGATTCT	50.0	50.0	58.0	57.8	803-808 bp	1.0	79.0
rbcL-accD_p3	GCTTGTGAAGTGTGGAAGGA	CTCGCCTGTGTATTCCGATT	50.0	50.0	58.0	57.8	805-810 bp	1.0	79.0
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TCGCCTGTGTATTCCGATTC	55.0	50.0	57.7	57.8	823-828 bp	1.0	77.0
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	CGCCTGTGTATTCCGATTCT	55.0	50.0	57.7	57.8	822-827 bp	1.0	76.9