Hotspot

atpB-rbcL

LSC · 53928-54715 · 788 bp

Back to Spryginia

Pi 0.00254

MarkerSeek score 60.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 3
pi: 0.00254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	876-890 bp	1.0	71.6
atpB-rbcL_p2	CGATACGTCCCAGGTTGTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	907-921 bp	1.0	71.4
atpB-rbcL_p3	CGAAACCTCTGGATCCGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	875-889 bp	1.0	71.3
atpB-rbcL_p4	CGATACGTCCCAGGTTGTTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	906-920 bp	1.0	71.1
atpB-rbcL_p5	ATCGAAACCTCTGGATCCGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	878-892 bp	1.0	70.3