Hotspot

trnH-CAC-psbA

LSC · 77-367 · 291 bp

Back to Spryginia

Pi 0.016

MarkerSeek score 63.10

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 291
variable_sites: 7
pi: 0.016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	349-368 bp	1.0	80.3
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	364-383 bp	1.0	80.1
trnH-CAC-psbA_p3	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	350-369 bp	1.0	80.1
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	367-386 bp	1.0	80.0
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	365-384 bp	1.0	79.9