Hotspot

rps15

SSC · 124942-125205 · 264 bp

Back to Sporoxeia

Pi 0.00758

MarkerSeek score 43.20

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 3
pi: 0.00758
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	ATCCCCCGTTGAAAATTCGT	45.0	45.0	58.1	57.8	832-858 bp	1.0	82.0
rps15_p2	TGCATTGATGGGTGATGAGG	ATCCCCCGTTGAAAATTCGT	50.0	45.0	57.9	57.8	825-851 bp	1.0	81.9
rps15_p3	GCATTGATGGGTGATGAGGT	ATCCCCCGTTGAAAATTCGT	50.0	45.0	57.9	57.8	824-850 bp	1.0	81.9
rps15_p4	TAACTGTTCGGTTTTCGGCA	ATCCCCCGTTGAAAATTCGT	45.0	45.0	58.0	57.8	938-964 bp	1.0	81.7
rps15_p5	TCTGTGCACCAATATCTGCC	ATCCCCCGTTGAAAATTCGT	50.0	45.0	58.0	57.8	1142-1168 bp	1.0	81.1