Hotspot

ndhE

SSC · 119433-119738 · 306 bp

Back to Sporoxeia

Pi 0.00436

MarkerSeek score 42.30

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 2
pi: 0.00436
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ACTCCTGGGTTGGCTAAGAT	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	897-904 bp	1.0	82.2
ndhE_p2	GCCTTACATCCATCCCAAGG	TTTGGACTGTTGGGAATGGG	55.0	50.0	58.0	58.0	1133-1140 bp	1.0	82.0
ndhE_p3	CGTGGAGCAGAAGCAATTTG	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	1157-1164 bp	1.0	81.9
ndhE_p4	ACCCATGCTTCGAGTTGTTT	TTTGGACTGTTGGGAATGGG	45.0	50.0	58.0	58.0	1260-1267 bp	1.0	81.7
ndhE_p5	GCTTCGAGTTGTTTCATGCC	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	1254-1261 bp	1.0	81.7