Hotspot

rps15-ycf1

SSC · 125206-125582 · 377 bp

Back to Sporoxeia

Pi 0.0213
MarkerSeek score 65.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
377
variable_sites
11
pi
0.0213
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCCCCGATTTTCTTCTTCTGA TGGAAGCCGTTTTAGTCTGT 42.9 45.0 56.7 57.1 461-486 bp 1.0 59.7
rps15-ycf1_p2 TCCCCGATTTTCTTCTTCTGA TGGAAGCCGTTTTAGTCTGTT 42.9 42.9 56.7 57.8 461-486 bp 1.0 57.4
rps15-ycf1_p3 TCCCCGATTTTCTTCTTCTGAA TGGAAGCCGTTTTAGTCTGT 40.9 45.0 57.4 57.1 461-486 bp 1.0 57.4
rps15-ycf1_p4 ATCCCCGATTTTCTTCTTCTGA TGGAAGCCGTTTTAGTCTGT 40.9 45.0 57.2 57.1 462-487 bp 1.0 55.7
rps15-ycf1_p5 TCCCCGATTTTCTTCTTCTGA ATGGAAGCCGTTTTAGTCTGT 42.9 42.9 56.7 57.6 462-487 bp 1.0 55.7