Hotspot

psaC-ndhE

SSC · 119193-119432 · 240 bp

Back to Sporoxeia

Pi 0.0307

MarkerSeek score 63.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 11
pi: 0.0307
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TGAGTACATCCTATACATGTATCA	GCGGCTATTGGGTTAGCTAT	33.3	50.0	54.2	57.5	368-374 bp	1.0	43.7
psaC-ndhE_p2	TCATAAATCTTTACTGAATGTGACA	TTATAGCTATTGCCGCAGCC	28.0	50.0	54.8	58.2	370-376 bp	1.0	43.6
psaC-ndhE_p3	TGAGTACATCCTATACATGTATCA	AGCGGCTATTGGGTTAGCTA	33.3	50.0	54.2	58.6	369-375 bp	1.0	43.6
psaC-ndhE_p4	TGAGTACATCCTATACATGTATCA	TTATAGCTATTGCCGCAGCC	33.3	50.0	54.2	58.2	391-397 bp	1.0	43.4
psaC-ndhE_p5	TGAGTACATCCTATACATGTATCAT	TTATAGCTATTGCCGCAGCC	32.0	50.0	54.7	58.2	391-397 bp	1.0	43.4