Hotspot

psbE-petL

LSC · 65478-66609 · 1132 bp

Back to Sporoxeia

Pi 0.00883

MarkerSeek score 51.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 15
pi: 0.00883
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATCGGCCATTTATCGTGCTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2433-2441 bp	1.0	80.9
psbE-petL_p2	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2172-2180 bp	1.0	80.9
psbE-petL_p3	CGGTAGGTACAGCTAGTCCA	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	2080-2088 bp	1.0	80.9
psbE-petL_p4	CTTCCTCCGCCATTTCATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2416-2424 bp	1.0	80.8
psbE-petL_p5	TCCTCCGCCATTTCATCAAG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2414-2422 bp	1.0	80.8