Hotspot

petA-psbJ

LSC · 63762-64670 · 909 bp

Back to Sporoxeia

Pi 0.0114

MarkerSeek score 62.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 15
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.6	58.1	1035-1055 bp	1.0	78.2
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.6	58.2	1039-1059 bp	1.0	77.0
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	TTCATACTCAGGGTTGGGCT	45.0	50.0	57.6	58.3	1038-1058 bp	1.0	76.3
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	GGTTCATACTCAGGGTTGGG	45.0	55.0	57.6	57.6	1040-1060 bp	1.0	75.6
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	ATACTCAGGGTTGGGCTCAT	50.0	50.0	57.2	58.1	1041-1061 bp	1.0	75.4