Hotspot

accD-psaI

LSC · 59550-60413 · 864 bp

Back to Sporoxeia

Pi 0.0166

MarkerSeek score 60.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 864
variable_sites: 21
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGTGTTCTGAGTGAGT	ACGGCACAAAAATAGAGGGT	45.0	45.0	56.3	57.4	936-969 bp	1.0	65.4
accD-psaI_p2	AAGGGTGTTCTGAGTGAGTT	ACGGCACAAAAATAGAGGGT	45.0	45.0	56.3	57.4	935-968 bp	1.0	65.4
accD-psaI_p3	AAAGGGTGTTCTGAGTGAGTT	ACGGCACAAAAATAGAGGGT	42.9	45.0	57.1	57.4	936-969 bp	1.0	63.6
accD-psaI_p4	AAAGGGTGTTCTGAGTGAGT	AACGGCACAAAAATAGAGGGT	45.0	42.9	56.3	58.1	937-970 bp	1.0	61.0
accD-psaI_p5	AAGGGTGTTCTGAGTGAGTT	AACGGCACAAAAATAGAGGGT	45.0	42.9	56.3	58.1	936-969 bp	1.0	61.0